A Biological Science Technician (Plants) position with USDA, ARS at the Tree Fruit Research Laboratory, Wenatchee, Washington State is posted at USAJOBS website with announcement # ARS-S21Y-11004543-AC.
This is a permanent position (GS-5/6/7$35,265.00 - $56,790 per annum) and requires US citizenship to apply. The primary duty is to assist research investigating molecular mechanisms underlying apple (or other tree crops) root resistance to soilborne pathogens. Recent graduates with a B.S. or M.S. degree in Plant Pathology, Plant Molecular Biology, Plant Science or other related majors are encouraged to apply. Knowledge and experience in plant molecular biology, plant-microbe interactions and tissue culture are highly desirable.
Specific knowledge and skills related to this position include: Following established protocols or modifying an existing protocol, a person with experience and skills in the following areas will be essential to carry out experiments: DNA/RNA isolation and quantification, PCR-based gene expression analysis, DNA cloning and plasmid construction, generating and maintaining transgenic plants, biochemical and enzymatic assays of immune responses or other phenotypes in transgenic plants. Knowledge and experience in applying common bioinformatic software for sequence analysis and/or gene expression data is a plus.
Experience or familiarity using the following lab instruments is desirable but not essential: tabletop centrifuge, nanodrop-1000, electric gel apparatus, regular PCR, BioRad CFX real-time PCR , Tecan Infinite M200 microplate reader.
Maximized exploitation of plant natural resistance to manage soilborne diseases, such as apple replant disease, is critical for reducing the soil input of chemical fumigants which negatively impact human health and environment. This research program is to understand the genetic controls and molecular mechanisms in roots of apple (or other tree fruit) as they are challenged with soilborne pathogens. Various functional genomics approaches are utilized to identify and validate specific apple genes for their regulatory roles contributing to disease resistance in apple root to soilborne necrotrophic replant pathogens.